Conjugation

Part:BBa_I714030:Design

Designed by: Mingzhi Qu   Group: iGEM07_Peking   (2007-10-20)


OriT-F (Origin of Transfer for the F-plasmid nic region)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 278
    Illegal NheI site found at 324
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This OriT-F is the same as the host cell's native Origin of Transfer, Please compare the sequence with J01002.


Source

Origin-of-transfer nic region excised from Plasmid F genomic DNA.(NCBI:AP001918)

References